1A3.3.13 (ATCC® HB-11894)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybri  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain:  BALB/c (B cell); BALB/c (myeloma)

Storage Conditions liquid nitrogen vapor phase
Images
Derivation
Animals were immunized with c-Met-IgG fusion protein (cellular receptor for HGF). Lymph node cells were fused with P3X63Ag8U.1 mouse myeloma cells (ATCC CRL-1597). The 1A3.3.13 antibody significantly blocked binding of HGF [5,686,292].
Genes Expressed
immunoglobulin; monoclonal antibody; against hepatocyte growth factor (HGF) receptor
Cellular Products
immunoglobulin; monoclonal antibody; against hepatocyte growth factor (HGF) receptor
Comments
Animals were immunized with c-Met-IgG fusion protein (cellular receptor for HGF). Lymph node cells were fused with P3X63Ag8U.1 mouse myeloma cells (ATCC CRL-1597). The 1A3.3.13 antibody significantly blocked binding of HGF [5,686,292].
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 x 10(6) cells/ml.
Interval: Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml.
Subcultivation Ratio: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with an additional 10% fetal bovine serum and 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype IgG1 kappa , IgG1; kappa light chain
Name of Depositor Genetech, Inc.
References

Schwall RH, Tabor KH. Hepatocyte growth factor receptor antagonist antibodies and uses thereof. US Patent 5,686,292 dated Nov 11 1997

Schwall RH, Tabor KH. Nucleic acids encoding hepatocyte growth factor receptor antagonist antibodies. US Patent 5,646,036 dated Jul 8 1997

Schwall RH, Tabor KH. Hepatocyte growth factor receptor antagonists and uses thereof. US Patent 6,468,529 dated Oct 22 2002

Basic Documentation
Other Documentation
References

Schwall RH, Tabor KH. Hepatocyte growth factor receptor antagonist antibodies and uses thereof. US Patent 5,686,292 dated Nov 11 1997

Schwall RH, Tabor KH. Nucleic acids encoding hepatocyte growth factor receptor antagonist antibodies. US Patent 5,646,036 dated Jul 8 1997

Schwall RH, Tabor KH. Hepatocyte growth factor receptor antagonists and uses thereof. US Patent 6,468,529 dated Oct 22 2002