MDTC-RP19 (ATCC® CRL-8135)

Organism: Meleagris gallopavo, turkey  / 

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Organism Meleagris gallopavo, turkey
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Comments
The line is free of major oncogenic avian viruses.
The cells are pathogenic in chickens but fluids are not.
The cells grow well at 41C.
Complete Growth Medium A 1:1 mixture of Leibovitz's L-15 medium and McCoy's 5a medium with 2 mM L-glutamine, 55%; tryptose phosphate broth, 5%; chicken serum, 20%; fetal bovine serum, 20% The fetal bovine serum concentration may be reduced to 10% after the cells have been in culture for 10 to 14 days.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium, 90%; DMSO, 10%
Culture Conditions
Temperature: 41.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor K Nazerian
References

Nazerian K, et al. Establishment of B-lymphoblastoid cell lines from Marek's disease virus- induced tumors in turkeys. Int. J. Cancer 29: 63-68, 1982. PubMed: 6277804

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Nazerian K, et al. Establishment of B-lymphoblastoid cell lines from Marek's disease virus- induced tumors in turkeys. Int. J. Cancer 29: 63-68, 1982. PubMed: 6277804