NCI-H841 [H841] (ATCC® CRL-5845)

Organism: Homo sapiens, human  /  Disease: stage L,carcinoma; variant small cell lung cancer

Permits and Restrictions

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Organism Homo sapiens, human
Product Format frozen
Culture Properties mixed: adherent and suspension, Cultures grow as floating aggregates of round cell clusters and as attached epithelial-like cells.
Biosafety Level 1
Disease stage L,carcinoma; variant small cell lung cancer
Age 51 years
Gender male
Ethnicity Caucasian, White
Applications
The line was established in October 1984.
Karyotype t(2p,3p), t(3q,9q)
Derivation
The line was established in October 1984.
Clinical Data
The patient received prior chemotherapy and radiation therapy.
The patient was a smoker.
male
Caucasian, White
51 years
Comments
The line was established in October 1984.
The patient received prior chemotherapy and radiation therapy.
The patient was a smoker.
70.
Complete Growth Medium HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Add fresh medium as cell density increases.
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium.
Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11
D13S317: 12
D16S539: 13
D5S818: 11,12
D7S820: 10,13
THO1: 6,9
TPOX: 11,12
vWA: 16,17
Name of Depositor AF Gazdar, JD Minna
Year of Origin 1984
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Basic Documentation
Restrictions

The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.