SVEC4-10EE2 (ATCC® CRL-2167)

Organism: Mus musculus, mouse  /  Cell Type: endothelial, SV40 transformed  / 

Organism Mus musculus, mouse
Cell Type endothelial, SV40 transformed
Product Format frozen
Morphology cobblestone
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences
Age adult
Gender male
Strain C3H/HeJ
Applications
SVEC4-10EE2 is an endothelial cell line derived from the same solid tumor as SVEC4-10EHR1 (see CRL-2161) in a nude mouse injected with SVEC4-10 (see ATCC CRL-2181).
The cells were cloned in 1989 by limiting dilution, and are distinguished by their inability to differentiate on a synthetic basement-like membrane.
The cells exhibit a stable cobblestone morphology in contrast to its sibling from the same tumor (SVEC4-10EHR1).
Storage Conditions liquid nitrogen vapor phase
Derivation
SVEC4-10EE2 is an endothelial cell line derived from the same solid tumor as SVEC4-10EHR1 (see CRL-2161) in a nude mouse injected with SVEC4-10 (see ATCC CRL-2181).
Clinical Data
male
Antigen Expression
H-2 K; Factor VIII related antigen; VCAM
Receptor Expression
high affinity receptors for low density lipoprotein (LDL)
Genes Expressed
H-2 K; Factor VIII related antigen; VCAM
Tumorigenic Yes
Effects
Yes, the cells induce spindle tumors with some of the histopathologic characteristics of human Kaposi Sarcoma after a latency period of approximately 14 weeks
Comments
SVEC4-10EE2 is an endothelial cell line derived from the same solid tumor as SVEC4-10EHR1 (see CRL-2161) in a nude mouse injected with SVEC4-10 (see ATCC CRL-2181).
The cells were cloned in 1989 by limiting dilution, and are distinguished by their inability to differentiate on a synthetic basement-like membrane.
They form tube-like structures in the presence of suramin, a drug which blocks growth factor receptors.
The cells exhibit a stable cobblestone morphology in contrast to its sibling from the same tumor (SVEC4-10EHR1).
The cells express the cell surface major histocompatibility complex class I antigen, H-2 k of the parental cell line, and express Factor VIII related antigen, high affinity receptors for low density lipoprotein and vascular cell adhesion molecule (VCAM).
The cells stain positively for SV40 T antigen.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
Subculturing
Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, rinse and remove trypsin. Add fresh trypsin solution (1 to 2 ml), and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor KA O'Connell
Year of Origin 1989
References

O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170

O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612

O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299

Basic Documentation
References

O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170

O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612

O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299