3B-11 (ATCC® CRL-2160)

Organism: Mus musculus, mouse  /  Cell Type: endothelial, SV40 transformed  / 

Organism Mus musculus, mouse
Cell Type endothelial, SV40 transformed
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences
Age adult
Gender male
Strain C3H/HeJ
Applications
The 3B-11 cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161).
They are resistant to lysis by activated macrophages as measured in the chromium release assay.
This clone retains the ability to differentiate on a synthetic basement-like membrane.
Storage Conditions liquid nitrogen vapor phase
Derivation
The 3B-11 cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161).
Clinical Data
male
Antigen Expression
H-2 K; VCAM
Genes Expressed
H-2 K; VCAM
Tumorigenic Yes
Effects
Yes, the cells induced spindle tumors with some histopathologic characteristics of human Kaposi Sarcoma after a shortened latency period of approximately 2 weeks
Comments
The 3B-11 cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161).
3B-11 cells were cloned in 1992 by limiting dilution.
They are resistant to lysis by activated macrophages as measured in the chromium release assay.
This clone retains the ability to differentiate on a synthetic basement-like membrane.
The cells express the cell surface major histocompatibility complex class I antigen, H-2 k, of the parental cell line, and express VCAM (vascular cell adhesion molecule).
The cells stain positively for SV40 T antigen.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor KA O'Connell
Year of Origin 1992
References

O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170

O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612

O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299

Basic Documentation
References

O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170

O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612

O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299