NCI-H2106 [H2106] (ATCC® CRL-5923™) Organism: Homo sapiens, human / Tissue: lung; derived from metastatic site: lymph node / Disease: stage 4, non-small cell lung cancer General Information Characteristics Culture Method History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits View Restrictions Organism Homo sapiens, human Tissue lung; derived from metastatic site: lymph node Product Format frozen Morphology floating clusters Biosafety Level 1 Disease stage 4, non-small cell lung cancer Age 58 years Gender male Ethnicity Caucasian Storage Conditions liquid nitrogen vapor phase Derivation The line was established in December 1988 from lymph node, a metastatic site. The patient received prior chemotherapy and radiation therapy. The tissue donor was a non-smoker. Clinical Data 58 years male Caucasian Complete Growth Medium HITES medium supplemented with 5% fetal bovine serumThe base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium 0.005 mg/ml Insulin 0.01 mg/ml Transferrin 30nM Sodium selenite (final conc.) 10 nM Hydrocortisone (final conc.) 10 nM beta-estradiol (final conc.) extra 2mM L-glutamine (for final conc. of 4.5 mM) 5% fetal bovine serum (final conc.) Subculturing Cultures can be maintained by the addition of fresh medium. Cells grow as floating aggregates of round cell clusters. Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density). Cryopreservation Culture medium with an additional 5% fetal bovine serum, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. Culture Conditions Temperature: 37°C Name of Depositor AF Gazdar, JD Minna Year of Origin 1988 References NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS Restrictions The line is available with the following restrictions: This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233. References NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.