NCI-H1869 [H1869] (ATCC® CRL-5900)

Organism: Homo sapiens, human  /  Cell Type: squamous cell  /  Disease: stage 4,non-small cell lung cancer

Permits and Restrictions

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Organism Homo sapiens, human
Cell Type squamous cell
Product Format frozen
Culture Properties adherent, adherent
Biosafety Level 1
Disease stage 4,non-small cell lung cancer
Age 58 years
Gender male
Ethnicity Caucasian
Applications
The line was established in March 1988.
Derivation
The line was established in March 1988.
Clinical Data
The patient received prior chemotherapy and radiation therapy.
The patient was a smoker.
male
Caucasian
58 years
Comments
The line was established in March 1988.
The patient received prior chemotherapy and radiation therapy.
The patient was a smoker.
50 pack years.
Complete Growth Medium ACL-4 medium supplemented with 10% FBS
The base medium for this cell line is ATCC-formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
  1. 0.02 mg/ml insulin
  2. 0.01 mg/ml transferrin
  3. 25 nM sodium selenite
  4. 50 nM Hydrocortisone
  5. 1 ng/ml Epidermal Growth Factor (do not filter)
  6. 0.01 mM ethanolamine
  7. 0.01 mM phosphorylethanolamine
  8. 100 pM triiodothyronine
  9. 0.5% (w/v) bovine serum albumin
  10. 10 mM HEPES
  11. 0.5 mM sodium pyruvate
  12. 2mM L-glutamine(for final conc. of 4.5 mM)
  13. 10% FBS

Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Centrifuge, resuspend cells in fresh medium and dispense into new flasks.
Cryopreservation
RPMI 1640 medium 85%; fetal bovine serum, 10%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 12
D5S818: 11
D7S820: 8,9
THO1: 6
TPOX: 8,9
vWA: 16
Name of Depositor AF Gazdar, JD Minna
Year of Origin 1988
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Basic Documentation
Restrictions

The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.