NCI-H1651 [H1651] (ATCC® CRL-5884)

Organism: Homo sapiens, human  /  Disease: adenocarcinoma; non-small cell lung cancer

Permits and Restrictions

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Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma; non-small cell lung cancer
Age 71 years
Gender male
Applications
The line was established in May 1987.
Storage Conditions liquid nitrogen vapor temperature
Derivation
The line was established in May 1987.
Clinical Data
The tissue donor was a non-smoker.
male
Comments
The line was established in May 1987.
The tissue donor was a non-smoker.
Complete Growth Medium ACL-4 medium supplemented with 10% FBS
The base medium for this cell line is ATCC-formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
  1. 0.02 mg/ml insulin
  2. 0.01 mg/ml transferrin
  3. 25 nM sodium selenite
  4. 50 nM Hydrocortisone
  5. 1 ng/ml Epidermal Growth Factor (do not filter)
  6. 0.01 mM ethanolamine
  7. 0.01 mM phosphorylethanolamine
  8. 100 pM triiodothyronine
  9. 0.5% (w/v) bovine serum albumin
  10. 10 mM HEPES
  11. 0.5 mM sodium pyruvate
  12. 2mM L-glutamine(for final conc. of 4.5 mM)
  13. 10% FBS

Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin- 0.53mM EDTA solution. Remove the solution and add an additional 2 to 3 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.4. Add 6.0 to 8.0 ml of complete growth medium containing 10% FBS and aspirate cells by gently pipetting4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new culture vessels.
Subcultivation Ratio: A subcultivation of 1:3 to 1:8 is recommended
Medium Renewal: Twice weekly
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 10,13
D13S317: 11
D16S539: 11
D5S818: 12,14
D7S820: 8
THO1: 8
TPOX: 11
vWA: 18
Name of Depositor AF Gazdar, JD Minna
Year of Origin 1987
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.