NCI-H23 [H23] (ATCC® CRL-5800)

Organism: Homo sapiens, human  /  Tissue: lung  /  Disease: adenocarcinoma; non-small cell lung cancer

Permits and Restrictions

View Permits View Restrictions

Organism Homo sapiens, human
Tissue lung
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma; non-small cell lung cancer
Age 51 years
Gender male
Ethnicity Black
Derivation

This line was derived from a lung cancer obtained from a patient prior to therapy.


Clinical Data
51 years
Black
male
This line was derived from a lung cancer obtained from a patient prior to therapy.
Oncogene myc +; src +; abl +; erb +; ras +; sis -
Genes Expressed
myc +; src +; abl +; erb +; ras +; sis -
Comments
The cells carry the K-ras 12 mutation, and there is a mutation in codon 246 (ATC -> ATG, isoleucine -> methionine) of the p53 gene.
There is expression of C-myc, L-myc, v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras and N-ras RNAs.
The cells express heterogeneous mRNA expression for PDGF A and B chain, transforming growth factor alpha and beta and the epidermal growth factor receptor (EGFR).
NCI-H23 exhibits a high degree of c-myc DNA amplification (20-fold) but no detectable amplification of c-myc RNA.
The cells stain positive for keratins 5+8 and 18 and vimentin but are negative for neurofilament.
NCI-H23 cells are L-dopa decarboxylase-negative.

They have a reported colony forming efficiency of 9.7% in soft agarose.


Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove spent medium, and add fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes at room temperature. Remove the trypsin and incubate the flask at 37°C for 5 to 10 minutes or until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 11
D5S818: 12,13
D7S820: 9,10
THO1: 6
TPOX: 8,9
vWA: 16,17
Population Doubling Time 38 hrs
Name of Depositor AF Gazdar, JD Minna
References

Little CD, et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. PubMed: 6646201

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

Mitsudomi T, et al. p53 gene mutations in non-small-cell lung cancer cell lines and their correlation with the presence of ras mutations and clinical features. Oncogene 7: 171-180, 1992. PubMed: 1311061

Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644

Broers JL, et al. Spontaneous changes in intermediate filament protein expression patterns in lung cancer cell lines. J. Cell Sci. 91: 91-108, 1988. PubMed: 2473086

Forsberg K, et al. Expression of functional PDGF beta receptors in a human large-cell lung- carcinoma cell line. Int. J. Cancer 53: 556-560, 1993. PubMed: 8382192

Gazdar AF, et al. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 6108156

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Lung Cancer 4: 155-161, 1988.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

References

Little CD, et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. PubMed: 6646201

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

Mitsudomi T, et al. p53 gene mutations in non-small-cell lung cancer cell lines and their correlation with the presence of ras mutations and clinical features. Oncogene 7: 171-180, 1992. PubMed: 1311061

Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644

Broers JL, et al. Spontaneous changes in intermediate filament protein expression patterns in lung cancer cell lines. J. Cell Sci. 91: 91-108, 1988. PubMed: 2473086

Forsberg K, et al. Expression of functional PDGF beta receptors in a human large-cell lung- carcinoma cell line. Int. J. Cancer 53: 556-560, 1993. PubMed: 8382192

Gazdar AF, et al. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 6108156

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Lung Cancer 4: 155-161, 1988.