AMJ2-C11 (ATCC® CRL-2456)

Organism: Mus musculus, mouse  /  Cell Type: macrophage (alveolar); infected with J2 virus  /  Tissue: lung  / 

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Organism Mus musculus, mouse
Tissue lung
Cell Type macrophage (alveolar); infected with J2 virus
Product Format frozen
Morphology macrophage
Culture Properties suspension, with some loosely adherent cells
Biosafety Level 2  [Cells contain J2 murine retrovirus viral DNA sequences]
Age 10 weeks old
Gender female
Strain C57BL/6J
Storage Conditions liquid nitrogen vapor phase
Derivation
AMJ2-C8 (ATCC CRL-2455) and AMJ2-C11 (ATCC CRL-2456) are cloned, continuous, alveolar macrophage (AM) cell lines generated from C57BL6J mice by in vitro infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes.
Clinical Data
female
10 weeks old
Antigen Expression
MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; Ly-5 +; Thy-1 -; Lyt-1 -
Genes Expressed
interleukin-6 (interleukin 6, IL-6)
Comments

AMJ2-C8 (ATCC CRL-2455) and AMJ2-C11 (ATCC CRL-2456) are cloned, continuous, alveolar macrophage (AM) cell lines generated from C57BL6J mice by in vitro infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes.

Flow cytometry detected the product of the raf gene in the cytoplasm of these cell lines.

Studies on the tumoricidal properties of these cell lines demonstrated differences in their response to a panel of known macrophage activators.

AMJ2-C8 was activated following exposure to recombinant murine interferon gamma (rMuIFN-gamma) but not lipopolysaccharide (LPS) or muramyl dipeptide (MDP).

AMJ2-C11 most closely resembled the response pattern of the parental AM, since it could be activated by either the combination of rMuIFN-gamma plus LPS or rMuIFN-gamma plus MDP.

The cells retain many characteristics of alveolar macrophages. They are phagocytic, non-specific esterase positive and they express macrophage Mac-1 antigens and Fc receptors.

Constitutive expression of MHC-class-II antigens was low on AMJ2-C11 but was increased following exposure to rMuIFN-gamma.

The cell line did not secrete substantial amounts of IL-1 or TNF but did secrete large amounts of IL-6.

The cells produce nitric oxide (NO) when stimulated with a mixture of rMuIFN-gamma and LPS.






Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 5 mM HEPES, 95%; fetal bovine serum, 5%
Subculturing Firmly tap the flask against palm of hand to dislodge any attached cells and transfer along with the floating cells into new flasks.

Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with an additional 5% fetal bovine serum and 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor AV Palleroni
References

Palleroni AV, et al. Tumoricidal alveolar macrophage and tumor infiltrating macrophage cell lines. Int. J. Cancer 49: 296-302, 1991. PubMed: 1879973

Palleroni AV, et al. Nitric oxide synthase induction in lines of macrophages from different anatomical sites. Cell. Mol. Biol. 44: 527-535, 1998. PubMed: 9620450

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Palleroni AV, et al. Tumoricidal alveolar macrophage and tumor infiltrating macrophage cell lines. Int. J. Cancer 49: 296-302, 1991. PubMed: 1879973

Palleroni AV, et al. Nitric oxide synthase induction in lines of macrophages from different anatomical sites. Cell. Mol. Biol. 44: 527-535, 1998. PubMed: 9620450