DMS 79 (ATCC® CRL-2049)

Organism: Homo sapiens, human  /  Tissue: lung/pleural fluid  /  Disease: carcinoma; small cell lung cancer

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Organism Homo sapiens, human
Tissue
lung/pleural fluid
Product Format frozen
Culture Properties suspension, multicell aggregates
Biosafety Level 1
Disease carcinoma; small cell lung cancer
Age 65 years
Ethnicity Caucasian
Storage Conditions liquid nitrogen phase
Karyotype Number of cells examined = 80; Modal Chromosome Number = 79 with a range of 65 to 84; Polyploidy Rate = 14.3%
Derivation
The line was established from cells in the pleural fluid of a patient with small cell carcinoma of the lung.
The patient had previously been treated with cytoxan, vincristine, methotrexate and radiation therapy.
Clinical Data
65 years
Caucasian
Antigen Expression
Leu 7; My23
Receptor Expression
epidermal growth factor (EGF)
Oncogene c-myc +; N-myc +; c-raf-1 +; H-ras +; K-ras +; N-ras +; v-fes -; v-fms -
Genes Expressed
adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; corticotropin; beta endorphin; 17 beta estradiol; lipotropin; oxytocin - neurophysin (OT-NP); parathormone; somatostatin-like immunoreactivity (SRIF)
Tumorigenic Yes
Effects
Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells.
Comments The cell express HLA class I and class II antigens.

Derivative chromosomes observed at high frequency in 24 karyotyped metaphases included: der(2)t(2;?18)(q10;q10); del(3)?(pter-->p21::q10-->qter); del(6)(q13); der(10); add(11)(p15); der(14)t(14;17)(q10;q10); add(15)(p11); add(15)(p11)+; and del(17)(?q24).

Two copies of each derivative chromosome was observed in each metaphase.
Less frequent derivative chromosomes included: del(12)?(q11q21)[18/24 cells] and add(13)(p11)[10/24 cells]. Four to eight additional marker chromosomes of unknown origin were generally detected in each cell.
Cytogenetics Comments: This is a hyper-triploid human cancer cell line with a modal chromosome number of 79.
A Y chromosome was verified by QM staining and C-banding. Normal chromosomes 10, 15 and 17 were absent.

The composite karyotype consists of 65 to 84, XY, +1, -2, der(2)t(2;?18)(q10;q10)(x2); -3, del(3)?(pter-->p21::q10-->qter)x2, -4, -5, +6, del(6)(?q15)x2,+7,+8,-9,-10,-10,-10, der(10)x2, -11, add(11)(p15)x2, -12, del(12)?(q11q21)[18], -13, +14, der(14)t(14;17)(q10;q10)x2, -15, -15,- 15, der(15)x2, -16, -17, -17, 17, del(17)x2, -18, +19, -20, +21, -22[cp24]

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL. Cell counts are approximate since the cells grow in aggregates.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 12
D5S818: 10
D7S820: 9,11
THO1: 8
TPOX: 8
vWA: 18
Name of Depositor OS Pettengill, G Sorenson
References

Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917

Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631

Pettengill OS, et al. Animal model for small cell carcinoma of the lung. Effect of immunosuppression and sex of mouse on tumor growth in nude athymic mice. Exp. Cell Biol. 48: 279-297, 1980. PubMed: 6249655

Lung Cancer 4: 155-161, 1988.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

Note: These cells are distributed subject to the following: 1.) This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College. 2.) Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. Tel: (603) 646-3675. 3.) In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications.

References

Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917

Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631

Pettengill OS, et al. Animal model for small cell carcinoma of the lung. Effect of immunosuppression and sex of mouse on tumor growth in nude athymic mice. Exp. Cell Biol. 48: 279-297, 1980. PubMed: 6249655

Lung Cancer 4: 155-161, 1988.