MH-S (ATCC® CRL-2019)

Organism: Mus musculus, mouse  /  Cell Type: macrophage (alveolar)  /  Tissue: lung  / 

Permits and Restrictions

View Permits

Organism Mus musculus, mouse
Tissue lung
Cell Type macrophage (alveolar)
Product Format frozen
Morphology macrophage
Culture Properties mixed, adherent and suspension
Biosafety Level 2    [Cells may contain SV40 virus]
Age 7 weeks
Gender male
Strain BALB/c
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
The MH-S cell line was derived by SV40 transformation of an adherent cell enriched population of mouse alveolar macrophages.
Clinical Data
male
7 weeks
Antigen Expression
CD11b (Mac-1); Class II antigens (I-A); T antigen
Receptor Expression
Fc

Genes Expressed interleukin 1 (IL-1)
Cellular Products
interleukin 1 (IL-1)
Comments The cells retain many of the properties of alveolar macrophages including typical macrophage morphology.
They are adherent, phagocytic, esterase positive and peroxidase negative.
Lipopolysaccharide (LPS) treatment stimulates IL-1 production.
The cells are capable of suppressing the in vitro plaque forming cell (PFC) response in a cell dose dependent manner.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by transferring floating cells to a centrifuge tube Rinse adherent cells with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add to floating cells collected above and centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Culture Conditions
Temperature: 37°C
Name of Depositor I Mbawuike, HB Herscowitz
References

Mbawuike IN, Hercowitz HB. MH-S, a murine alveolar macrophage cell line: morphological, cytochemical, and functional characteristics. J. Leukocyte Biol. 46: 119-127, 1989. PubMed: 2787372

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Mbawuike IN, Hercowitz HB. MH-S, a murine alveolar macrophage cell line: morphological, cytochemical, and functional characteristics. J. Leukocyte Biol. 46: 119-127, 1989. PubMed: 2787372