CHL/IU [CHL-11] (ATCC® CRL-1935)

Organism: Cricetulus griseus, hamster, Chinese  / 

Permits and Restrictions

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Organism Cricetulus griseus, hamster, Chinese
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age newborn
Gender female
Applications
This line was derived from the CHL line established by Utakoji in 1970.
The line is useful for screening chemical for ability to induce chromosomal aberrations.
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 25
Derivation
This line was derived from the CHL line established by Utakoji in 1970.
Clinical Data
female
Comments
This line was derived from the CHL line established by Utakoji in 1970.
The line is useful for screening chemical for ability to induce chromosomal aberrations.
Complete Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; bovine calf serum, 10%
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended
Medium Renewal: 1 to 2 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Population Doubling Time 15 hrs
Name of Depositor M Ishidate
Year of Origin 1970
References

Data book of chromosomal aberration test in vitro. New York: Elsevier; 1988.

Ishidate M Jr., Odashima S. Chromosome tests with 134 compounds on Chinese hamster cells in vitro-- a screening for chemical carcinogens. Mutat. Res. 48: 337-354, 1977. PubMed: 876270

Koyama H, et al. A new cell line derived from newborn Chinese hamster lung tissue. Gann 61: 161-167, 1970. PubMed: 5452405

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Data book of chromosomal aberration test in vitro. New York: Elsevier; 1988.

Ishidate M Jr., Odashima S. Chromosome tests with 134 compounds on Chinese hamster cells in vitro-- a screening for chemical carcinogens. Mutat. Res. 48: 337-354, 1977. PubMed: 876270

Koyama H, et al. A new cell line derived from newborn Chinese hamster lung tissue. Gann 61: 161-167, 1970. PubMed: 5452405