NZP-12 (ATCC® CRL-1921)

Organism: Crossarchus obscurus, Cusimanse  / 

Permits and Restrictions

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Organism Crossarchus obscurus, Cusimanse
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Comments
The cells can be propagated for at least 13 population doublings beyond frozen reference seed stock aspirate and dispense into new flasks.
Complete Growth Medium Dulbecco's modified Eagle's medium, 45%; Ham's F12 medium, 45%; fetal bovine serum, 10%; plus the following additives: 10 ng/ml epidermal growth factor; 0.005 mg/ml insulin; 5 ng/ml selenium; 0.005 mg/ml transferrin
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Allow the flask to sit at room temperature, or incubate at 37C, until the cells detach (about 5 to 10 minutes). Add fresh medium.
Name of Depositor L Munson
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation