NZP-12 (ATCC® CRL-1921)

Organism: Crossarchus obscurus, Cusimanse  / 

Organism Crossarchus obscurus, Cusimanse
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Comments
The cells can be propagated for at least 13 population doublings beyond frozen reference seed stock aspirate and dispense into new flasks.
Complete Growth Medium Dulbecco's modified Eagle's medium, 45%; Ham's F12 medium, 45%; fetal bovine serum, 10%; plus the following additives: 10 ng/ml epidermal growth factor; 0.005 mg/ml insulin; 5 ng/ml selenium; 0.005 mg/ml transferrin
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Allow the flask to sit at room temperature, or incubate at 37C, until the cells detach (about 5 to 10 minutes). Add fresh medium.
Name of Depositor L Munson