CCD-34Lu (ATCC® CRL-1491)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

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Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 2.5 weeks
Gender female
Ethnicity Black
Applications
The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility).
A normal skin line derived from the same patient is available as ATCC CRL-1497.
Derivation
The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility).
A normal skin line derived from the same patient is available as ATCC CRL-1497.
Clinical Data
The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility).
A normal skin line derived from the same patient is available as ATCC CRL-1497.
female
Black
2.5 weeks
Comments
The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility).
A normal skin line derived from the same patient is available as ATCC CRL-1497.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days.
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 11,13
D13S317: 9,13
D16S539: 8,13
D5S818: 12
D7S820: 10
THO1: 7,9
TPOX: 11
vWA: 15,16
Name of Depositor GP Maxwell
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation