CCD-33Lu (ATCC® CRL-1490)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  /  Tissue: lung  / 

Permits and Restrictions

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Organism Homo sapiens, human
Tissue lung
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 7 years
Gender male
Ethnicity Caucasian
The line was derived from normal lung tissue from a child who died of a brain tumor.
Clinical Data
7 years
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37°C for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11
D16S539: 11,13
D5S818: 11,12
D7S820: 11,13
THO1: 6,9.3
TPOX: 9,11
vWA: 15,16
Name of Depositor GP Maxwell
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation