KLN 205 (ATCC® CRL-1453)

Organism: Mus musculus, mouse  /  Tissue: lung  /  Disease: squamous cell carcinoma

Organism Mus musculus, mouse
Tissue
lung
Product Format frozen
Culture Properties adherent
Biosafety Level 1
Disease squamous cell carcinoma
Strain DBA/2
Storage Conditions lquid nitrogen vapor phase
Tumorigenic Yes
Effects
Yes, in DBA/2 and BDF1 mice
Comments
KLN 205 cells form metastatic lesions in lungs after inoculation into mice.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: lquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor T Kaneko
References

Kaneko T, LePage GA. Growth characteristics and drug responses of a murine lung carcinoma in vitro and in vivo. Cancer Res. 38: 2084-2090, 1978. PubMed: 418873

Basic Documentation
References

Kaneko T, LePage GA. Growth characteristics and drug responses of a murine lung carcinoma in vitro and in vivo. Cancer Res. 38: 2084-2090, 1978. PubMed: 418873