RL-65 (ATCC® CRL-10354)

Organism: Rattus norvegicus, rat  /  Cell Type: Squamous Epithelium,Epithelial  / 

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Organism Rattus norvegicus, rat
Cell Type Squamous Epithelium,Epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age 5 days
Strain Sprague-Dawley
Applications
The cells exhibit different characteristic when grown with or without retinoic acid.
In the presence of retinoic acid (50 nM), the cells resemble low non-keratinized or squamous epithelium with densely packed colonies.
Genes Expressed
cytoskeletal proteins (alpha keratin, actin, desmin, vimentin, tubulin); fibronectin; laminin
Tumorigenic No
Effects
No, in immunosuppressed mice
Yes, in semisolid medium.
Comments
The cells exhibit different characteristic when grown with or without retinoic acid.
In the presence of retinoic acid (50 nM), the cells resemble low non-keratinized or squamous epithelium with densely packed colonies.
In the absence of retinoic acid, the cells form a keratinized epithelium.
Long term cultures in the absence of retinoic acid form a stratified highly keratinized epithelium with large networks of epithelial filaments.
The cells are maintained in a serum free medium.
If grown in media containing serum, the properties of the cells will change.
Complete Growth Medium A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 2.5 mM L-glutamine adjusted to contain 1.2 g/L sodium bicarbonate and supplemented with 0.005 mg/ml insulin, 0.01 mg/ml human transferrin, 0.1 mM ethanolamine, 0.1 mM phosphoethanolamine, 25 nM selenium, 500 nM hydrocortisone, 0.005 mM forskolin, and bovine pituitary extract (0.15 mg protein per ml).
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin - 0.53 mM EDTA, rinse and remove trypsin. Allow the culture to sit at room temperature (or 37C) until the cells detach (about 5 minutes).
Centrifuge, resuspend cells in fresh medium and dispense into new flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor Genentech, Inc.
References

Mather JP, Roberts PE. Method of isolating lung cell line. US Patent 5,364,785 dated Nov 15 1994

Roberts PE, et al. A novel epithelial cell from neonatal rat lung: isolation and differentiated phenotype. Am. J. Physiol. 259: L415-L425, 1990. PubMed: 2260675

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Mather JP, Roberts PE. Method of isolating lung cell line. US Patent 5,364,785 dated Nov 15 1994

Roberts PE, et al. A novel epithelial cell from neonatal rat lung: isolation and differentiated phenotype. Am. J. Physiol. 259: L415-L425, 1990. PubMed: 2260675