DBS-FRhL-2 (ATCC® CL-160)

Organism: Macaca mulatta, monkey, rhesus  /  Cell Type: fibroblast  /  Tissue: lung  / 

Organism Macaca mulatta, monkey, rhesus
Tissue lung
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age fetus
Gender male
Storage Conditions liquid nitrogen vapor phase
Karyotype Model number = 42. Range = 37 to 46.Seventy percent of the cells are diploid and the karyotype is that of a male rhesus monkey. No structural abnormalities are evident.
Clinical Data
male
Tumorigenic No
Effects
No, in immunosuppressed mice
Yes, in semisolid medium.
no; The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Virus Susceptibility Human poliovirus 1
Human poliovirus 2
Human poliovirus 3
Human Coxsackievirus A 9
Parainfluenza 3
Rubella virus , Rubella virus
Mumps virus
Vaccinia virus
Vesicular stomatitis, Orsay (Indiana)
Vesicular stomatitis, Glasgow (Indiana)
Human rhinovirus 2
Comments
This line was formerly designated CCL-160.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. Add appropriate aliquots of the cell suspension to new culture vessels. 
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
Name of Depositor U.S. Government
Year of Origin April, 1969
References

Hopps HE, et al. Sub-human primate diploid cell lines as substrates for virus vaccine production. US Patent 4,040,905 dated Aug 9 1977

Wallace RE, et al. Development of a diploid cell line from fetal rhesus monkey lung for virus vaccine production. In Vitro 8: 323-332, 1973. PubMed: 4348695

Basic Documentation
References

Hopps HE, et al. Sub-human primate diploid cell lines as substrates for virus vaccine production. US Patent 4,040,905 dated Aug 9 1977

Wallace RE, et al. Development of a diploid cell line from fetal rhesus monkey lung for virus vaccine production. In Vitro 8: 323-332, 1973. PubMed: 4348695