Tb 1 Lu (ATCC® CCL-88)

Organism: Tadarida brasiliensis, bat, free-tailed  / 

Organism Tadarida brasiliensis, bat, free-tailed
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age adult
Gender female
Storage Conditions liquid nitrogen vapor pahse
Karyotype modal number = 48; range = 42 to 53
Polyploidy about 1%. Dicentric chromosomes or chromosomes with chromatids fused at distal end in 6% of cells.
Clinical Data
female
Virus Resistance
adenovirus 5; coxsackievirus A9, B5; herpes simplex; bovine diarrhea; infectious bovine rhinotracheitis; poliovirus 2
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor pahse
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor AJ Kniazeff, D Constantine, WA Nelson-Rees, D Schmidt
Year of Origin October, 1965