MLg [Mlg 2908] (ATCC® CCL-206)

Organism: Mus musculus, mouse  /  Tissue: lung  /  Disease: normal

Organism Mus musculus, mouse
Tissue lung
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease normal
Age newborn
Strain ddY
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 64; range = 51 to 76.
The stemline chromosome number is hypertriploid with 2S component occurring at 2.4%. All chromosomes were acrocentric. Usually there was a small acrocentric chromosome per S cell genome that was composed of a large centromeric heterochromatin (based on C-banding analysis) and a small segment of euchromatin. No Y-like chromosome(s) was detected (based on Q- and C-bandings).
Comments
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor Y Hirokawa
Year of Origin January, 1964
References

Yoshikura H, Hirokawa Y. Endogenous C-type virus of a mouse cell line and its defectiveness. J. Virol. 13: 1319-1325, 1974. PubMed: 4364904

Basic Documentation
References

Yoshikura H, Hirokawa Y. Endogenous C-type virus of a mouse cell line and its defectiveness. J. Virol. 13: 1319-1325, 1974. PubMed: 4364904