CCD-13Lu (ATCC® CCL-200)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  /  Disease: Carcinoma

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Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease Carcinoma
Age 71 years
Gender male
Ethnicity Black
Applications
Derived from normal tissue from a patient with pancreatic carcinoma.
Karyotype normal human male; diploid; stable
Derivation
Derived from normal tissue from a patient with pancreatic carcinoma.
Clinical Data
Derived from normal tissue from a patient with pancreatic carcinoma.
male
Black
71 years
Comments
Derived from normal tissue from a patient with pancreatic carcinoma.
The cells senesce at about population doubling level (PDL) 19.
The current stock is at PDL 7.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Remove culture medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit for 10 to 12 minutes at 37C. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Isoenzymes
G6PD, A
Name of Depositor MI Cour
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation