HLF-a (ATCC® CCL-199)

Organism: Homo sapiens, human  /  Disease: epidermoid carcinoma

Organism Homo sapiens, human
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease epidermoid carcinoma
Age 54 years
Gender female
Ethnicity Black
Storage Conditions liquid nitrogen vapor phase
Karyotype human female; diploid; stable
Clinical Data
female
Black
54 years
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 12,13
D5S818: 8,12
D7S820: 9,12
THO1: 6,9.3
TPOX: 6,8
vWA: 15
Isoenzymes
G6PD, B
Name of Depositor GD Stoner
Year of Origin February, 1976
References

This fibroblast-like cell line was derived from the peripheral lung tissue of a patient epidermoid carcinoma of the lung. The line was initiated in February, 1976 by G. Stoner using tissue explants taken away from the region of the carcinoma.

Basic Documentation
References

This fibroblast-like cell line was derived from the peripheral lung tissue of a patient epidermoid carcinoma of the lung. The line was initiated in February, 1976 by G. Stoner using tissue explants taken away from the region of the carcinoma.