HLF-a (ATCC® CCL-199)

Organism: Homo sapiens, human  /  Disease: epidermoid carcinoma

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Organism Homo sapiens, human
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease epidermoid carcinoma
Age 54 years
Gender female
Ethnicity Black
Storage Conditions liquid nitrogen vapor phase
Karyotype human female; diploid; stable
Clinical Data
female
Black
54 years
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 12,13
D5S818: 8,12
D7S820: 9,12
THO1: 6,9.3
TPOX: 6,8
vWA: 15
Isoenzymes
G6PD, B
Name of Depositor GD Stoner
Year of Origin February, 1976
References

This fibroblast-like cell line was derived from the peripheral lung tissue of a patient epidermoid carcinoma of the lung. The line was initiated in February, 1976 by G. Stoner using tissue explants taken away from the region of the carcinoma.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

This fibroblast-like cell line was derived from the peripheral lung tissue of a patient epidermoid carcinoma of the lung. The line was initiated in February, 1976 by G. Stoner using tissue explants taken away from the region of the carcinoma.