DBS-FCL-2 (ATCC® CCL-162)

Organism: Cercopithecus aethiops  / 

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Organism Cercopithecus aethiops
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 141 days
Gender male
Karyotype normal male; diploid; stable
Clinical Data
male
Tumorigenic No
Effects
No, in immunosuppressed mice
Yes, in semisolid medium
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin for several minutes and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor U.S. Government
References

Hopps HE, et al. Sub-human primate diploid cell lines as substrates for virus vaccine production. US Patent 4,040,905 dated Aug 9 1977

Wallace RE, et al. Development and characterization of cell lines from subhuman primates. In Vitro 8: 333-341, 1973. PubMed: 4633070

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hopps HE, et al. Sub-human primate diploid cell lines as substrates for virus vaccine production. US Patent 4,040,905 dated Aug 9 1977

Wallace RE, et al. Development and characterization of cell lines from subhuman primates. In Vitro 8: 333-341, 1973. PubMed: 4633070