AK-D (ATCC® CCL-150)

Organism: Felis catus, cat  / 

Organism Felis catus, cat
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age fetus
Complete Growth Medium Ham's F12K medium, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA for 3 to 5 minutes, remove trypsin, EDTA leaving enough to wet the cell sheet and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor A Kniazeff
References

. . Lab. Invest. 34: 494-500, 1976.

Basic Documentation
References

. . Lab. Invest. 34: 494-500, 1976.