L2 (ATCC® CCL-149)

Organism: Rattus norvegicus, rat  /  Tissue: lung  / 

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Organism Rattus norvegicus, rat
Tissue lung
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age adult
Gender female
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 77; range = 49 to 85. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Clinical Data
female
Comments
Gamma glutamylcysteine synthetase activity is stimulated by oxidative stress.
Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor ME Kaighn
References

Shi MM, et al. Quinone-induced oxidative stress elevates glutathione and induces gamma- glutamylcysteine synthetase activity in rat lung epithelial L2 cells. J. Biol. Chem. 269: 26512-26517, 1994. PubMed: 7929374

Douglas WH, Kaighn ME. Clonal isolation of differentiated rat lung cells. In Vitro 10: 230-237, 1974. PubMed: 4616002

Su WY, et al. Lung epithelial cell-released nitric oxide protects against PMN-mediated cell injury. Am. J. Physiol. 271: L581-L586, 1996. PubMed: 8897905

Pei L. Identification of a negative glucocorticoid response element in the rat type 1 vasoactive intestinal polypeptide receptor gene. J. Biol. Chem. 271: 20879-20884, 1996. PubMed: 8702844

Yang J, et al. Identification of an 11-residue portion of CTP-phosphocholine cytidylyltransferase that is required for enzyme-membrane interactions. Biochem. J. 325: 29-38, 1997. PubMed: 9224626

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Shi MM, et al. Quinone-induced oxidative stress elevates glutathione and induces gamma- glutamylcysteine synthetase activity in rat lung epithelial L2 cells. J. Biol. Chem. 269: 26512-26517, 1994. PubMed: 7929374

Douglas WH, Kaighn ME. Clonal isolation of differentiated rat lung cells. In Vitro 10: 230-237, 1974. PubMed: 4616002

Su WY, et al. Lung epithelial cell-released nitric oxide protects against PMN-mediated cell injury. Am. J. Physiol. 271: L581-L586, 1996. PubMed: 8897905

Pei L. Identification of a negative glucocorticoid response element in the rat type 1 vasoactive intestinal polypeptide receptor gene. J. Biol. Chem. 271: 20879-20884, 1996. PubMed: 8702844

Yang J, et al. Identification of an 11-residue portion of CTP-phosphocholine cytidylyltransferase that is required for enzyme-membrane interactions. Biochem. J. 325: 29-38, 1997. PubMed: 9224626