BNL 1NG A.2 (ATCC® TIB-76)

Organism: Mus musculus, mouse  /  Cell Type: chemically transformed  / 

Organism Mus musculus, mouse
Cell Type chemically transformed
Product Format frozen
Culture Properties adherent
Biosafety Level 1
Age embryo
Strain BALB/c
Applications
This line was derived from BNL CL.2 (ATCC TIB-73) by transformation with nitrosoguanidine.
Tested and found negative for ectromelia virus (mousepox).
Derivation
This line was derived from BNL CL.2 (ATCC TIB-73) by transformation with nitrosoguanidine.
Tumorigenic Yes
Effects
Yes, in BALB/c mice and ATxFL mice
Comments
This line was derived from BNL CL.2 (ATCC TIB-73) by transformation with nitrosoguanidine.
The cells will grow in 0.3% agarose.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Twice per week
Remove old medium, add fresh 0.25% trypsin for 1 minute, remove trypsin and let the culture sit at room temperature until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor P Patek, J Collins, M Cohn
References

Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934

Basic Documentation
References

Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934