c37 (B7IFi1) (ATCC® CRL-2711)

Organism: Mus musculus, mouse  /  Cell Type: epithelial  /  Disease: hepatoma

Organism Mus musculus, mouse
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease hepatoma
Strain C57L/J
Applications
ICR191G (a frame shift mutagen) was used to treat Hepa-1c1c7 cells.
The line may be used to study xenobiotic (and carcinogenic) metabolism in the absence of cytochrome P4501A1 activity, which is known to metabolize benzo[a]pyrene cytotoxic and carcinogenic intermediates.
The c37 (B7IFi1) cell line is one of these, and lacks cytochrome P4501A1 dependent aryl hydrocarbon hydroylase (AHH) activity due to two point mutations in the CYP1A1 protein (Leu-118 to Arg and Arg-245 to Pro).
The c37 (B7IFi1) cell line was derived from Hepa-1c1c7 (ATCC CRL-2026).
Storage Conditions liquid nitrogen vapor phase
Derivation
The c37 (B7IFi1) cell line was derived from Hepa-1c1c7 (ATCC CRL-2026). Hepa-1c1c7 has high aryl hydrocarbon hydroxylase (AHH) activity. ICR191G (a frame shift mutagen) was used to treat Hepa-1c1c7 cells. Mutated colonies were selected for benzo[a]pyrene resistance. The c37 (B7IFi1) cell line is one of these, and lacks cytochrome P4501A1 dependent aryl hydrocarbon hydroylase (AHH) activity due to two point mutations in the CYP1A1 protein (Leu-118 to Arg and Arg-245 to Pro). The line may be used to study xenobiotic (and carcinogenic) metabolism in the absence of cytochrome P4501A1 activity, which is known to metabolize benzo[a]pyrene cytotoxic and carcinogenic intermediates. It is also a tool to study the putative natural ligand for the induction of this enzyme.
Comments
The c37 (B7IFi1) cell line was derived from Hepa-1c1c7 (ATCC CRL-2026). Hepa-1c1c7 has high aryl hydrocarbon hydroxylase (AHH) activity. ICR191G (a frame shift mutagen) was used to treat Hepa-1c1c7 cells. Mutated colonies were selected for benzo[a]pyrene resistance. The c37 (B7IFi1) cell line is one of these, and lacks cytochrome P4501A1 dependent aryl hydrocarbon hydroylase (AHH) activity due to two point mutations in the CYP1A1 protein (Leu-118 to Arg and Arg-245 to Pro). The line may be used to study xenobiotic (and carcinogenic) metabolism in the absence of cytochrome P4501A1 activity, which is known to metabolize benzo[a]pyrene cytotoxic and carcinogenic intermediates. It is also a tool to study the putative natural ligand for the induction of this enzyme.
Complete Growth Medium Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides with 2 mM L-glutamine, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor O Hankinson
References

Hankinson O. Single-step selection of clones of a mouse hepatoma line deficient in aryl hydrocarbon hydroxylase. Proc. Natl. Acad. Sci. USA 76: 373-376, 1979. PubMed: 106390

Kimura S, et al. Analysis of two benzo[a]pyrene-resistant mutants of the mouse hepatoma Hepa-1 P(1)450 gene via cDNA expression in yeast. EMBO J. 6: 1929-1933, 1987. PubMed: 3308449

The c37 (B7IFi1) cell line was derived from Hepa-1c1c7 (ATCC CRL-2026). Hepa-1c1c7 has high aryl hydrocarbon hydroxylase (AHH) activity. ICR191G (a frame shift mutagen) was used to treat Hepa-1c1c7 cells. Mutated colonies were selected for benzo[a]pyrene resistance. The c37 (B7IFi1) cell line is one of these, and lacks cytochrome P4501A1 dependent aryl hydrocarbon hydroylase (AHH) activity due to two point mutations in the CYP1A1 protein (Leu-118 to Arg and Arg-245 to Pro). The line may be used to study xenobiotic (and carcinogenic) metabolism in the absence of cytochrome P4501A1 activity, which is known to metabolize benzo[a]pyrene cytotoxic and carcinogenic intermediates. It is also a tool to study the putative natural ligand for the induction of this enzyme.

Basic Documentation
References

Hankinson O. Single-step selection of clones of a mouse hepatoma line deficient in aryl hydrocarbon hydroxylase. Proc. Natl. Acad. Sci. USA 76: 373-376, 1979. PubMed: 106390

Kimura S, et al. Analysis of two benzo[a]pyrene-resistant mutants of the mouse hepatoma Hepa-1 P(1)450 gene via cDNA expression in yeast. EMBO J. 6: 1929-1933, 1987. PubMed: 3308449

The c37 (B7IFi1) cell line was derived from Hepa-1c1c7 (ATCC CRL-2026). Hepa-1c1c7 has high aryl hydrocarbon hydroxylase (AHH) activity. ICR191G (a frame shift mutagen) was used to treat Hepa-1c1c7 cells. Mutated colonies were selected for benzo[a]pyrene resistance. The c37 (B7IFi1) cell line is one of these, and lacks cytochrome P4501A1 dependent aryl hydrocarbon hydroylase (AHH) activity due to two point mutations in the CYP1A1 protein (Leu-118 to Arg and Arg-245 to Pro). The line may be used to study xenobiotic (and carcinogenic) metabolism in the absence of cytochrome P4501A1 activity, which is known to metabolize benzo[a]pyrene cytotoxic and carcinogenic intermediates. It is also a tool to study the putative natural ligand for the induction of this enzyme.