Sl/Sl4 hSCF220 (ATCC® CRL-2453)

Organism: Mus musculus, mouse  /  Cell Type: Mast Cell  / 

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Organism Mus musculus, mouse
Cell Type Mast Cell
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 2 Papovavirus (Cells may contain SV40 viral sequences)
Age embryo
Applications
SL/Sl4 hSCF220 is a transfected SL/Sl4 cell line that expresses membrane-bound stem cell factor (SCF).
This cell line was derived from the Sl/Sl4 cell line (ATCC CRL-2452) by transfection with spliced forms of hSCF cDNAs inserted into the pJT-1 (hSCF220) vector and expressed off the simian virus 40 (SV40) early promoter.
Defects in the HM, associated with the Steel (Sl) mutation in mice, have been shown to be due to abnormalities in the production or presentation of the protein product of the Steel gene.
Sl/Sl4 is a SV40 large T antigen immortalized stromal cell line derived from the hematopoietic microenvironment (HM) of a fetal murine homozygous (Sl/Sl) SCF-deficient embryo.
It exists as a locally secreted or membrane-bound protein.
SL/Sl4 hSCF220 cells do not secrete detectable amounts of SCF.
Secreted SCF is available in the transfected Sl/Sl4 cell line, Sl/Sl4 hSCF248 (ATCC CRL-2354).
Storage Conditions liquid nitrogen vapor phase
Derivation
This cell line was derived from the Sl/Sl4 cell line (ATCC CRL-2452) by transfection with spliced forms of hSCF cDNAs inserted into the pJT-1 (hSCF220) vector and expressed off the simian virus 40 (SV40) early promoter.
Sl/Sl4 is a SV40 large T antigen immortalized stromal cell line derived from the hematopoietic microenvironment (HM) of a fetal murine homozygous (Sl/Sl) SCF-deficient embryo.
Comments
SL/Sl4 hSCF220 is a transfected SL/Sl4 cell line that expresses membrane-bound stem cell factor (SCF).
This cell line was derived from the Sl/Sl4 cell line (ATCC CRL-2452) by transfection with spliced forms of hSCF cDNAs inserted into the pJT-1 (hSCF220) vector and expressed off the simian virus 40 (SV40) early promoter.
It was cotransfected with the p48 vector encoding hygromycin B using the Lipofectin method.
Defects in the HM, associated with the Steel (Sl) mutation in mice, have been shown to be due to abnormalities in the production or presentation of the protein product of the Steel gene.
Sl/Sl4 is a SV40 large T antigen immortalized stromal cell line derived from the hematopoietic microenvironment (HM) of a fetal murine homozygous (Sl/Sl) SCF-deficient embryo.
This product is termed stem cell factor (SCF) or mast cell growth factor (MGF). It exists as a locally secreted or membrane-bound protein.
Both forms show biological activity when assayed on human hematopoietic cells.
SL/Sl4 hSCF220 cells that express the membrane-bound form (hSCF220) of SCF supported the maintenance of human progenitor cells in culture for up to four weeks This was 1-2 weeks longer than SL/Sl4 hSCF248.
SL/Sl4 hSCF220 cells do not secrete detectable amounts of SCF.
Secreted SCF is available in the transfected Sl/Sl4 cell line, Sl/Sl4 hSCF248 (ATCC CRL-2354).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:30 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Culture vessels must be coated with 0.1% gelatin.
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor DA Williams
References

Toksoz D, et al. Support of human hematopoiesis in long-term bone marrow cultures by murine stromal cells selectively expressing the membrane-bound and secreted forms of the human homolog of the steel gene product, stem cell factor. Proc. Natl. Acad. Sci. USA 89: 7350-7354, 1992. PubMed: 1380155

Majumdar MK, et al. Identification and mutation of primary and secondary proteolytic cleavage sites in murine stem cell factor cDNA yields biologically active, cell-associated protein. J. Biol. Chem. 269: 1237-1242, 1994. PubMed: 7507105

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Toksoz D, et al. Support of human hematopoiesis in long-term bone marrow cultures by murine stromal cells selectively expressing the membrane-bound and secreted forms of the human homolog of the steel gene product, stem cell factor. Proc. Natl. Acad. Sci. USA 89: 7350-7354, 1992. PubMed: 1380155

Majumdar MK, et al. Identification and mutation of primary and secondary proteolytic cleavage sites in murine stem cell factor cDNA yields biologically active, cell-associated protein. J. Biol. Chem. 269: 1237-1242, 1994. PubMed: 7507105