SOB-15 (ATCC® CRL-2301)

Organism: Oncorhynchus mykiss, trout, rainbow  /  Cell Type: epithelial  / 

Organism Oncorhynchus mykiss, trout, rainbow
Cell Type epithelial
Product Format frozen
Morphology spindle-like with polygonal foci
Culture Properties adherent
Biosafety Level 1
Strain Shasta
Storage Conditions liquid nitrogen vapor phase
Comments
The cells retain characteristics of normal fish liver
Complete Growth Medium Minium essential Medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate and supplemented with 2 mM L-glutamine, 1.32 g/L sodium chloride, 0.1 mM non-essential amino acids and 10% heat-inactivated fetal bovine serum.
Subculturing
Protocol: Remove the spent medium, rinse flask with fresh 0.25% trypsin, 0.03% EDTA and remove trypsin, Add an additional 1 to 2 ml of trypsin solution, and allow the flask to sit at room temperature until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
The cells should be grown in PRIMARIA flasks (Falcon Nos. 3813 or 3824)
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 17.0°C; (Min. 16.0 °C, Max. 18.0°C)
Name of Depositor GK Ostrander, JB Blair
References

Ostrander GK, et al. Long-term primary culture epithelia cells from rainbow trout (Oncorhynchus mykiss) liver. In Vitro Cell. Dev. Biol. Anim. 31: 367-378, 1995. PubMed: 7543343

Basic Documentation
References

Ostrander GK, et al. Long-term primary culture epithelia cells from rainbow trout (Oncorhynchus mykiss) liver. In Vitro Cell. Dev. Biol. Anim. 31: 367-378, 1995. PubMed: 7543343