RTH-149 (ATCC® CRL-1710)

Organism: Oncorhynchus mykiss, trout, rainbow  /  Disease: hepatoma

Permits and Restrictions

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Organism Oncorhynchus mykiss, trout, rainbow
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease hepatoma
Storage Conditions liquid nitrogen vapor temperature
Comments
Culture the cells at 21C.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature until the cells detach (about 5 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 21.0°C
Name of Depositor JL Fryer
References

Lannan CN, et al. Fish cell lines: establishment and characterization of nine cell lines from salmonids. In Vitro 20: 671-676, 1984. PubMed: 6542066

. . Fish Pathol. 15: 193-200, 1981.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Lannan CN, et al. Fish cell lines: establishment and characterization of nine cell lines from salmonids. In Vitro 20: 671-676, 1984. PubMed: 6542066

. . Fish Pathol. 15: 193-200, 1981.