N1-S1 (ATCC® CRL-1604)

Organism: Rattus norvegicus, rat  /  Tissue: liver  /  Disease: Hepatoma; Novikoff hepatoma

Organism Rattus norvegicus, rat
Tissue liver
Product Format frozen
Culture Properties suspension
Biosafety Level 1
Disease Hepatoma; Novikoff hepatoma
Gender male
Strain Sprague-Dawley
Storage Conditions liquid nitrogen vapor phase
Derivation
The N1-S1 cell line was established from a hepatoma induced by feeding 4-dimethylaminoazobenzene to a male rat.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 104 viable cells/mL and subculture at 1 to 1.5 X 106 cells/mL (after about 3 days). Excessive clumping of cells can often be cured by trypsinizing the cells to disperse them.
Cryopreservation
Complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Name of Depositor JE Becker