McA-RH8994 (ATCC® CRL-1602)

Organism: Rattus norvegicus, rat  /  Tissue: liver  /  Disease: hepatoma; Morris hepatoma 8994

Permits and Restrictions

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Organism Rattus norvegicus, rat
Tissue liver
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease hepatoma; Morris hepatoma 8994
Gender male
Storage Conditions liquid nitrogen vapor phase
Clinical Data
male
Receptor Expression
glucocorticoid
Genes Expressed
alpha-fetoprotein (AFP)
Cellular Products
alpha-fetoprotein (AFP); alpha fetoprotein
Comments Addition of glucocorticoids induces alpha fetoprotein synthesis and slows cell proliferation.

AFP is also synthesized when cell growth slows as the cells end the log phase of growth.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 75%; horse serum, 20%; fetal bovine serum, 5%.
Subculturing
Remove medium, add fresh 0.25% trypsin solution, remove trypsin immediately and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture once per week.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 or 3 days
Name of Depositor JE Becker
References

Morris H, Meranze D. Induction and Some Characteristics of “Minimal Deviation” and Other Transplantable Rat Hepatomas. Recent Results Cancer Res. 44: 103-114, 1974.

Becker JE, et al. Two new rat hepatoma cell lines for studying the unbalanced blocked ontogeny hypothesisIn: Becker JE, et al. Onco-developmental gene expression. New YorkAcademic Press, pp. 259-270, 1976

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Morris H, Meranze D. Induction and Some Characteristics of “Minimal Deviation” and Other Transplantable Rat Hepatomas. Recent Results Cancer Res. 44: 103-114, 1974.

Becker JE, et al. Two new rat hepatoma cell lines for studying the unbalanced blocked ontogeny hypothesisIn: Becker JE, et al. Onco-developmental gene expression. New YorkAcademic Press, pp. 259-270, 1976