SQMK-FP (ATCC® CRL-2762)

Organism: Saimiri boliviensis boliviensis, monkey, bolivian squirrel  /  Cell Type: epithelial  / 

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Organism Saimiri boliviensis boliviensis, monkey, bolivian squirrel
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age 1 day newborn
Gender male
Applications
The cell line can be used to study the biology of the squirrel monkey in vitro.
The cells exhibit elevated expression of the FK506-binding immunophilin FKBP51. FKBP51 is the common cause of glucocorticoid resistance typical of the squirrel monkey.
SQMK-FP is a continuous, tubular, kidney epithelial cell line derived from a newborn squirrel monkey.
Storage Conditions liquid nitrogen vapor phase
Karyotype hyperdiploid with chromosome numbers ranging from 52 to 57 (submetacentric chromosome pair 15 and acrocentric chromosome pair 16). RefScammell JG, et al. A kidney epithelial cell line from a bolivian squirrel monkey. In Vitro Cell. Dev. Biol. Anim. 38: 258-261, 2002. PubMed: 12418921
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Derivation
SQMK-FP is a continuous, tubular, kidney epithelial cell line derived from a newborn squirrel monkey. Normal kidney tissue was minced and cultured. Proliferating cells were subsequently cloned by the limiting dilution. SQMK-FP cells have cell junctions with radiating filaments, indicating desmosomes and numerous surface projections containing longitudinally oriented filaments typical of tubular epithelium. The cells exhibit elevated expression of the FK506-binding immunophilin FKBP51. FKBP51 is the common cause of glucocorticoid resistance typical of the squirrel monkey. The cell line can be used to study the biology of the squirrel monkey in vitro.
Clinical Data
male
Comments
SQMK-FP is a continuous, tubular, kidney epithelial cell line derived from a newborn squirrel monkey. Normal kidney tissue was minced and cultured. Proliferating cells were subsequently cloned by the limiting dilution. SQMK-FP cells have cell junctions with radiating filaments, indicating desmosomes and numerous surface projections containing longitudinally oriented filaments typical of tubular epithelium. The cells exhibit elevated expression of the FK506-binding immunophilin FKBP51. FKBP51 is the common cause of glucocorticoid resistance typical of the squirrel monkey. The cell line can be used to study the biology of the squirrel monkey in vitro.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
    An inoculum of 5 X 10(4) to 1 X 10(5) viable cells/cm2 is recommended. Do not exceed 3 X 10(6) cells/cm2.
  6. Incubate cultures at 37°C.
Interval: Maintain cultures at a cell concentration between 1 X 10(5) and 2 X 10(6) cells/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every two to three days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Population Doubling Time approximately 22 hours
Name of Depositor JG Scammell
Year of Origin 1997
References

Scammell JG, et al. A kidney epithelial cell line from a bolivian squirrel monkey. In Vitro Cell. Dev. Biol. Anim. 38: 258-261, 2002. PubMed: 12418921

Scammell JG, et al. A kidney epithelial cell line from a bolivian squirrel monkey. In Vitro Cell. Dev. Biol. Anim. 38: 258-261, 2002. PubMed: 12418921

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
Restrictions

This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit

References

Scammell JG, et al. A kidney epithelial cell line from a bolivian squirrel monkey. In Vitro Cell. Dev. Biol. Anim. 38: 258-261, 2002. PubMed: 12418921

Scammell JG, et al. A kidney epithelial cell line from a bolivian squirrel monkey. In Vitro Cell. Dev. Biol. Anim. 38: 258-261, 2002. PubMed: 12418921