CCD 1105 KIDTr (ATCC® CRL-2305)

Organism: Homo sapiens, human  /  Cell Type: human papillomavirus 16 (HPV-16) E6/E7 transformed  / 

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Organism Homo sapiens, human
Cell Type human papillomavirus 16 (HPV-16) E6/E7 transformed
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 Cells contain Human Papilloma (HPV16) sequences
Age 133 days gestation
Applications
Passage 3 cells were transformed with a retrovirus vector (LXSN16E6E7 produced by ATCC CRL-2203) in the presence of polybrene.
The transformed cells are resistant to G418 and sensitive to Hygromycin B.
E6E7 sequences were detected by PCR in cells at passage 15.
Major Histocompatibility Complex class I or II molecules were not expressed on these cells, but PCR analyses revealed presence of the genes for directing synthesis of HLA antigens.
CCD 1105 KIDTr is sensitive to Coxsackieviruses A9 and B5, measles virus, poliovirus 1 and rhinovirus.
Derivation
Passage 3 cells were transformed with a retrovirus vector (LXSN16E6E7 produced by ATCC CRL-2203) in the presence of polybrene.
Oncogene E6/E7 +
Genes Expressed
keratin; acid phosphatase,E6/E7 +
Cellular Products
keratin; acid phosphatase
Comments
Fetal kidney was digested with collagenase: trypsin mixture. The digestion products were plated in REGM (Renal Epithelial Cell Growth Medium which include 0.5% FBS) from Clonetics on collagen l-fibronectin-BSA coated flasks.
Passage 3 cells were transformed with a retrovirus vector (LXSN16E6E7 produced by ATCC CRL-2203) in the presence of polybrene.
The transformed cells are resistant to G418 and sensitive to Hygromycin B.
This line stains positively with antibody for cytokeratin and is acid phosphatase positive.
After 50 population doublings, the cells continue dividing and retain cuboidal morphology.
E6E7 sequences were detected by PCR in cells at passage 15.
Major Histocompatibility Complex class I or II molecules were not expressed on these cells, but PCR analyses revealed presence of the genes for directing synthesis of HLA antigens.
CCD 1105 KIDTr is sensitive to Coxsackieviruses A9 and B5, measles virus, poliovirus 1 and rhinovirus.
Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor V Zabrenetzky, A Thompson
Passage History
Passage 3 cells were transformed with a retrovirus vector (LXSN16E6E7 produced by ATCC CRL-2203) in the presence of polybrene.
E6E7 sequences were detected by PCR in cells at passage 15.
References

Zabrenetzky V, et al. The isolation, immortalization and characterization of human fetal keratinocytes. In Vitro Cell. Dev. Biol. 33: Part II, p. 34A, 1997.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

Note: These cells are distributed for research purposes only. The American Type Culture Collection (ATCC) releases the line subject to the following: 1. CCD 1105 KIDTr cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of the ATCC; 2. Any proposed commercial use of this cell line must first be negotiated with the ATCC, P.O. 1549, Manassas VA 20108, telephone 703-365-2704, FAX 703-365-2730

References

Zabrenetzky V, et al. The isolation, immortalization and characterization of human fetal keratinocytes. In Vitro Cell. Dev. Biol. 33: Part II, p. 34A, 1997.