KNRK (ATCC® CRL-1569)

Organism: Rattus norvegicus, rat  /  Cell Type: Kirsten murine sarcoma virus (Ki-MSV) transformed  / 

Organism Rattus norvegicus, rat
Cell Type Kirsten murine sarcoma virus (Ki-MSV) transformed
Product Format frozen
Culture Properties mixed: adherent and suspension
Biosafety Level 1
Applications
The cells do not produce Ki-MSV particles.
Tested and found negative for ectromelia virus (mousepox).
Genes Expressed
The cells release growth factors in serum free medium.
Cellular Products
The cells release growth factors in serum free medium.
Comments
The cells do not produce Ki-MSV particles.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 95%; bovine calf serum, 5%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Cultures can be maintained by transferring floating cells to additional flasks.
Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA.
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor JE DeLarco
References

Aaronson SA, Weaver CA. Characterization of murine sarcoma virus (Kirsten) transformation of mouse and human cells. J. Gen. Virol. 13: 245-252, 1971. PubMed: 4333712

Gimona M, et al. Forced expression of tropomyosin 2 or 3 in v-Ki-ras-transformed fibroblasts results in distinct phenotypic effects. Proc. Natl. Acad. Sci. USA 93: 9618-9623, 1996. PubMed: 8790379

Bohm SK, et al. Mechamisms of desensitization and resensitization of proteinase-activated receptor-2. J. Biol. Chem. 271: 22003-22016, 1996. PubMed: 8703006

Basic Documentation
References

Aaronson SA, Weaver CA. Characterization of murine sarcoma virus (Kirsten) transformation of mouse and human cells. J. Gen. Virol. 13: 245-252, 1971. PubMed: 4333712

Gimona M, et al. Forced expression of tropomyosin 2 or 3 in v-Ki-ras-transformed fibroblasts results in distinct phenotypic effects. Proc. Natl. Acad. Sci. USA 93: 9618-9623, 1996. PubMed: 8790379

Bohm SK, et al. Mechamisms of desensitization and resensitization of proteinase-activated receptor-2. J. Biol. Chem. 271: 22003-22016, 1996. PubMed: 8703006