RK13 (ATCC® CCL-37)

Organism: Oryctolagus cuniculus, rabbit  /  Tissue: kidney  /  Disease: normal

Permits and Restrictions

View Permits

Organism Oryctolagus cuniculus, rabbit
Tissue
kidney
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 [Cells contain Bovine Viral Diarrhea Virus (BVDV)]
Disease normal
Age 5 weeks
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
keratin
Virus Susceptibility Herpes simplex virus
Pseudorabies virus
Vaccinia virus
Rabbitpox virus
Myxoma virus
Simian adenovirus 11
Simian adenovirus 17
Simian adenovirus 3
Simian adenovirus 1
Simian adenovirus 20
Simian adenovirus 18
Simian adenovirus 16
Simian adenovirus 8
Simian adenovirus 19
Simian adenovirus 21
Simian adenovirus 25
Simian adenovirus 22
Simian adenovirus 23
Simian adenovirus 38
Simian adenovirus 37
Simian adenovirus 27
Simian adenovirus 27
Simian adenovirus 39
Simian adenovirus 32
Simian adenovirus 34
Simian adenovirus 31
Simian adenovirus 33
Simian adenovirus 36
Rubella virus , Rubella virus
Comments

The cells are positive for keratin by immunoperoxidase staining. The cells are positive for bovine viral diarrhea virus (BVDV).

This cell line is susceptible to infection by the following viruses: B virus; herpes simplex; pseudorabies virus; vaccinia; rabbitpox; myxoma; simian adenoviruses; rubellavirus

Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor BC Meyer
References

Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708

Beale AJ, et al. Rabbit Cells Susceptible to Rubella Virus. The Lancet 282: 640-641 1963

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

McCarthy K, et al. Isolation of Rubella Virus from Cases in Britain. The Lancet 282: 593-598 1963. PubMed: 14050876

Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708

Beale AJ, et al. Rabbit Cells Susceptible to Rubella Virus. The Lancet 282: 640-641 1963

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

McCarthy K, et al. Isolation of Rubella Virus from Cases in Britain. The Lancet 282: 593-598 1963. PubMed: 14050876

Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321