MPK (ATCC® CCL-166)

Organism: minipig  /  Disease: Pneumonitis

Permits and Restrictions

View Permits

Organism minipig
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease Pneumonitis
Age 118 days gestation
Gender male
Clinical Data
male
Virus Resistance
poliovirus 1
Comments
Cells are refractory to infection by Chlamydia trachomatis strains TW-3, Bour and LGV 440L and Chlamydia psittaci strains 6BC and meningopneumonitis.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor HM Jenkin
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation