A6 (ATCC® CCL-102)

Organism: Xenopus laevis, frog, South African clawed  /  Tissue: kidney  / 

Organism Xenopus laevis, frog, South African clawed
Tissue
kidney
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age adult
Gender male
Clinical Data
male
Virus Resistance
poliovirus 1; vesicular stomatitis (Indiana); herpes simplex; vaccinia; pseudorabies
Complete Growth Medium NCTC 109 medium, 75%; distilled water, 15%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 26°C
Name of Depositor KA Rafferty
References

Mizell, M. Biology of amphibian tumors. New York: Springer-Verlag; 1969.

Rokaw MD, et al. Regulation of a sodium channel-associated G-protein by aldosterone. J. Biol. Chem. 271: 4491-4496, 1996. PubMed: 8626803

Basic Documentation
References

Mizell, M. Biology of amphibian tumors. New York: Springer-Verlag; 1969.

Rokaw MD, et al. Regulation of a sodium channel-associated G-protein by aldosterone. J. Biol. Chem. 271: 4491-4496, 1996. PubMed: 8626803