FHs 74 Int (ATCC® CCL-241)

Organism: Homo sapiens, human  /  Tissue: small intestine  / 

Organism Homo sapiens, human
Tissue small intestine
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age 3 to 4 months gestation
Gender female
Storage Conditions liquid nitrogen vapor phase
Karyotype normal female; diploid
Clinical Data
female
Receptor Expression
epidermal growth factor (EGF), expressed
Tumorigenic No
Effects
No, in immunosuppressed mice
Yes, in semisolid medium
Comments
PAS, negative.
The cells are negative for keratin by immunoperoxidase staining.
Complete Growth Medium Hybri-Care Medium ATCC 46-X supplemented with 30 ng/ml epidermal growth factor (EGF), 90%; fetal bovine serum, 10%
(Do not filter the medium after addition of EGF)
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

    Medium Renewal: Every 2 to 3 days
    Cryopreservation
    Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage Temperature: liquid nitrogen vapor phase
    Culture Conditions
    Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37°C
    STR Profile
    Amelogenin: X
    CSF1PO: 10,11
    D13S317: 11,12
    D16S539: 11,12
    D5S818: 11,12
    D7S820: 9,10
    THO1: 7,9.3
    TPOX: 8
    vWA: 16,18
    Isoenzymes
    AK-1, 1
    ES-D, 1-2
    G6PD, B
    GLO-I, 1
    Me-2, 1-2
    PGM1, 1
    PGM3, 1
    Name of Depositor R Owens
    References

    Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412

    Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258

    Smith HS, et al. Nuclear ultrastructure of epithelial cell lines derived from human carcinomas and nonmalignant tissues. Cancer Res. 39: 332-334, 1979. PubMed: 761205

    Basic Documentation
    References

    Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412

    Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258

    Smith HS, et al. Nuclear ultrastructure of epithelial cell lines derived from human carcinomas and nonmalignant tissues. Cancer Res. 39: 332-334, 1979. PubMed: 761205