WERI-Rb-1 (ATCC® HTB-169)

Organism: Homo sapiens, human  /  Cell Type: retinoblastoma  /  Tissue: eye, retina  /  Disease: retinoblastoma

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Organism Homo sapiens, human
Tissue eye, retina
Cell Type retinoblastoma
Product Format frozen
Morphology grape-like clusters of round cells
Culture Properties suspension
Biosafety Level 1
Disease retinoblastoma
Age 1 year
Gender female
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a near diploid line. The modal chromosome number is 47 occurring at 38%, and the rate of polyploidy is 9%. Fifteen to sixteen marker chromosomes are present in all cells. They are t(1,?), t(3p,5q), der(3)t(?q29;?), t(3q,?), 5q+, i(6p), t(7q,?), 9q+, t(10q,21q), 16q+ and five to six others. Normal chromosomes 3, 10, 13 and 16 are absent. There are two copies of the X chromosome. No Y chromosomes were detected in QM stained preparations
Derivation
The WERI-Rb-1 line is one of two human retinoblastoma cell lines established in 1974 by R.M. McFall and T.W. Sery.
Clinical Data
1 year
Caucasian
female
Tumorigenic Yes
Effects
Yes, in rabbits
Comments
The cells survive culturing in Difco Bacto-Agar but do not form colonies.
Scanning electron microscopy reveals some variation in the number and frequency of surface blebs, lamellipodia and microvilli.
The line is of interest in studies of cell differentiation, animal models of tumor therapy and biochemical evaluations.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 - 3 X 105 viable cells/mL. Maintain cell density between 1 X 105 and 1 to 2 X 106 viable cells/mL.
Medium Renewal: Every 3 to 4 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 37°C
Isoenzymes
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 0
Name of Depositor TW Sery
Year of Origin 1974
References

McFall RC, et al. Characterization of a new continuous cell line derived from a human retinoblastoma. Cancer Res. 37: 1003-1010, 1977. PubMed: 844036

McFall RC, et al. Scanning electron microscopic observation of two retinoblastoma cell lines. Cancer Res. 38: 2827-2835, 1978. PubMed: 679190

Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

McFall RC, et al. Characterization of a new continuous cell line derived from a human retinoblastoma. Cancer Res. 37: 1003-1010, 1977. PubMed: 844036

McFall RC, et al. Scanning electron microscopic observation of two retinoblastoma cell lines. Cancer Res. 38: 2827-2835, 1978. PubMed: 679190

Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024