HRT-18G (ATCC® CRL-11663)

Organism: Homo sapiens, human  / 

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Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age 67 years
Gender male
Applications
They can be used for propagation and plaque titration of bovine coronaviruses.
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is a derivative of HRT-18 and represents a subpopulation of cells selected by successive passage in different media.
Clinical Data
male
Comments
This line is a derivative of HRT-18 (ATCC CCL-244) and represents a subpopulation of cells selected by successive passage in different media.
HRT-18G cells show enhanced susceptibility to bovine coronaviruses relative to the parental HRT-18 cell line.
They can be used for propagation and plaque titration of bovine coronaviruses.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Cryopreservation
culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Name of Depositor Louisiana State Univ. Agricultural and Mechanical College
Passage History
This line is a derivative of HRT-18 and represents a subpopulation of cells selected by successive passage in different media.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation