NCI-H498 [H498] (ATCC® CCL-254)

Organism: Homo sapiens, human  /  Disease: colorectal adenocarcinoma

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Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties suspension, with some loosely adherent cells
Biosafety Level 1
Disease colorectal adenocarcinoma
Age 56 years
Gender male
Ethnicity Caucasian
Applications
This line was derived from a metastasis in the peritoneum obtained from a patient prior to therapy.
The cells form glandular structures and secrete mucin.
The cells are positive for Dopa decarboxylase, CA19-9 and TAG-72 antigens and CEA.
Although the patient from which this cell was derived was blood type A, the cell line does not react with anti type B antibody and has very low to undetectable levels of alpha-N-acetylgalactosaminyltransferase activity.
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 51; range = 47 to 54.
This is a hyperdiploid human cell line. The modal chromosome number is 51, occurring in 42% of the cells. The rate of higher ploidies is 5%. The 2q+ is the only marker chromosome detected and common to all cells. There are four copies for N7; 3 copies each for N8, N13 and N22, and a single copy for the X.
Derivation
This line was derived from a metastasis in the peritoneum obtained from a patient prior to therapy.
Although the patient from which this cell was derived was blood type A, the cell line does not react with anti type B antibody and has very low to undetectable levels of alpha-N-acetylgalactosaminyltransferase activity.
Clinical Data
This line was derived from a metastasis in the peritoneum obtained from a patient prior to therapy.
Although the patient from which this cell was derived was blood type A, the cell line does not react with anti type B antibody and has very low to undetectable levels of alpha-N-acetylgalactosaminyltransferase activity.
male
Caucasian
56 years
Antigen Expression
Rh+; CA19-9 antigen; TAG-72 antigen
Genes Expressed
This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil.
carcinoembryonic antigen (CEA), 826 ng/ml per 10 exp6 cells per 10 days; mucin,Rh+; CA19-9 antigen; TAG-72 antigen,The cells are positive for Dopa decarboxylase, CA19-9 and TAG-72 antigens and CEA.
Tumorigenic Yes
Effects
Yes, in nude mice
Comments
This line was derived from a metastasis in the peritoneum obtained from a patient prior to therapy.
The cells form glandular structures and secrete mucin.
The cells are positive for Dopa decarboxylase, CA19-9 and TAG-72 antigens and CEA.
Although the patient from which this cell was derived was blood type A, the cell line does not react with anti type B antibody and has very low to undetectable levels of alpha-N-acetylgalactosaminyltransferase activity.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Alternatively, the cells may be collected by centrifugation and then resuspended at 2 to 4 X 10(4) viable cells/ml. When the cell concentration becomes heavy with large clumps, aspirate well and and dilute approximately 1:10. If the clumps are allowed to get too large, most of the cells will die.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 9,11
D16S539: 9,11
D5S818: 13
D7S820: 8,11
THO1: 7
TPOX: 8
vWA: 15
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1-2
Name of Depositor AF Gazdar
References

Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249

Dahiya R, et al. ABH blood group antigen expression, synthesis, and degradation in human colonic adenocarcinoma cell lines. Cancer Res. 49: 4550-4556, 1989. PubMed: 2545345

Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249

Dahiya R, et al. ABH blood group antigen expression, synthesis, and degradation in human colonic adenocarcinoma cell lines. Cancer Res. 49: 4550-4556, 1989. PubMed: 2545345

Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells