NCI-H747 [H747] (ATCC® CCL-252)

Organism: Homo sapiens, human  /  Tissue: cecum  /  Disease: colorectal adenocarcinoma

Permits and Restrictions

View Restrictions

Organism Homo sapiens, human
Tissue cecum
Product Format frozen
Morphology epithelial
Culture Properties adherent, floating aggregates of round cells with some attached cells
Biosafety Level 1
Disease colorectal adenocarcinoma
Age 69 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 66; range = 63 to 75
This is a hypotriploid human cell line. The modal chromosome number is 66, occurring in 20% of cells. The rate of higher ploidies is 1.6%. Twelve marker chromosomes are common to all cells; these include single copy for i(1q), i(7p), i(10q); two copies for i(3q) and two to three copies for der(12)t(12;13)(q24,33;q12). The N12, N13, X and Y have single copy each. The presence of the Y was confirmed by Q-banding.
Derivation
This line was derived from a metastasis in a common duct node from a patient prior to therapy.
Clinical Data
69 years
Caucasian
male
Antigen Expression Blood type A; Rh+; CA19-9 antigen
Genes Expressed
carcinoembryonic antigen (CEA), 8 ng/mL per 106 cells per 10 days
Cellular Products
carcinoembryonic antigen (CEA), 8 ng/ml per 10 exp6 cells per 10 days
Tumorigenic Yes
Effects

Yes, in nude mice

Tumors developed in 3 of 5 nude mice inoculated subcutaneously with 107 cells

 

Comments

The cells are negative for Dopa decarboxylase and TAG-72 antigen but are positive for CEA and CA19-9 antigen.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1
Name of Depositor AF Gazdar
References

Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249

Basic Documentation
Restrictions

The line is available with the following restrictions:

  1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied.
  2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.

References

Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249