GPC-16 (ATCC® CCL-242)

Organism: Cavia porcellus, guinea pig  /  Disease: colorectal adenocarcinoma

Organism Cavia porcellus, guinea pig
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease colorectal adenocarcinoma
Applications
The cells are positive for keratin by immunoperoxidase staining.
Genes Expressed
The cells are negative for keratin by immunoperoxidase staining.
keratin,The cells are positive for keratin by immunoperoxidase staining.
Cellular Products
keratin
Tumorigenic Yes
Effects
Yes, in nude mice
Comments
PAS, positive.
The cells are positive for keratin by immunoperoxidase staining.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor RW O'Donnell
References

O'Donnell RW, Cockerell GL. Establishment and biological properties of a guinea pig colonic adenocarcinoma cell line induced by N-methyl-N-nitrosourea. Cancer Res. 41: 2372-2377, 1981. PubMed: 7237435

Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells

Basic Documentation
References

O'Donnell RW, Cockerell GL. Establishment and biological properties of a guinea pig colonic adenocarcinoma cell line induced by N-methyl-N-nitrosourea. Cancer Res. 41: 2372-2377, 1981. PubMed: 7237435

Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells