HeLa NR1 (ATCC® CRL-13011)

Organism: Homo sapiens, human  /  Cell Type: HeLa cells transfected with plasmid pSV2neoNR101  /  Disease: adenocarcinoma

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Organism Homo sapiens, human
Cell Type HeLa cells transfected with plasmid pSV2neoNR101
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 cells contain papova and SV40 viral DNA sequences
Disease adenocarcinoma
Age 31 years
Gender female
Ethnicity Black
Storage Conditions liquid nitrogen vapor temperature
Images
Clinical Data
female
Black
31 years
HeLa Markers Y
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.5 mg/ml G418 and 10% fetal bovine serum
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: 1:6 to 1:10
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Name of Depositor United States Government - DHHS
References

Parker JE, Kiel JL. Nitrate reductase-transfected HeLa cells for cancer and microwave bioeffects research. US Patent 6,326,196 dated Dec 4 2001

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Parker JE, Kiel JL. Nitrate reductase-transfected HeLa cells for cancer and microwave bioeffects research. US Patent 6,326,196 dated Dec 4 2001