MDA-MB-330 (ATCC® HTB-127)

Organism: Homo sapiens, human  / 

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Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age 43 years
Gender female
Ethnicity Caucasian
Applications
The population exhibited a mixed phenotype when examined by immunofluorescence staining for tubulin, with some cells (type I) having extensive arrays and others (type II) a more diffuse reaction pattern.
Karyotype modal number = 64 with one marker chromosome
Images
Clinical Data
female
Caucasian
43 years
Antigen Expression
Blood Type A; Rh+
Receptor Expression
epidermal growth factor (EGF)
Tumorigenic No
Effects
No, in immunosuppressed mice
Yes, in semisolid medium
Comments
The population exhibited a mixed phenotype when examined by immunofluorescence staining for tubulin, with some cells (type I) having extensive arrays and others (type II) a more diffuse reaction pattern.
Most cells of the line stained positively for actin.
This line grows very slowly (doubling time is 10 to 20 days) and never becomes confluent.
Complete Growth Medium These cells are grown in Leibovitz's L-15 medium with 2 mM L-glutamine supplemented with:
  • 30 ng/ ml human recombinant EGF
  • 0.016 mg/ml human recombinant insulin
  • 2mM glutathione
  • 20% fetal bovine serum
Do not filter EGF thru 0.22 micron filter.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Once per week
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 to 2 minutes, remove trypsin and let the culture sit at 37C until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 100%
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 9
D16S539: 9,12
D5S818: 13
D7S820: 10,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14,16
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 1
PGM3, 1
Population Doubling Time 240 to 480 hrs
Name of Depositor R Cailleau
References

Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.

Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
Restrictions

The ATCC Material is available with the following restrictions: The ATCC Material may not be used for commercial purposes. Purchaser will not make commercial use of the ATCC Material without obtaining a license from the University of Texas MD Anderson Cancer Center (“UTMDACC”). With respect to research use, prior to purchase, for-profit or commercial entities must first obtain a research use license from UTMDACC. For instructions on how to proceed, please contact Dustin J. Romine, M.A. at the UTMDACC Office of Technology Commercialization via email at dromine@mdanderson.org.

References

Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.

Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202