JC (ATCC® CRL-2116)

Organism: Mus musculus, mouse  /  Cell Type: Epithelial-like  /  Tissue: mammary gland  /  Disease: adenocarcinoma

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Organism Mus musculus, mouse
Tissue mammary gland
Cell Type Epithelial-like
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease adenocarcinoma
Age 1.5 years
Gender Female
Strain BALB/cRos
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 40; range = 26 to 100
Derivation
JC is an epithelial-like cell line established in 1983 from a spontaneous primary mammary adenocarcinoma along the milk line.
Clinical Data
female
The original tumor was passaged once in female BALB/c mice, and the resulting tumors were harvested and passaged in vitro for six months.
Tumorigenic Yes
Effects
Yes, produces tumors with papillary adenocarcinoma morphology in BALB/c mice
Comments
Electron microscopic examination detected the presence of cellular tonofilaments and desmosomes.
No type B or C virus particles were detected, although intracisternal A particles were observed occasionally.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution and remove trypsin. Add fresh trypsin solution and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Cryopreservation
Culture medium, 95%; DMSO, 5%
Population Doubling Time 12 to 16 hrs
Name of Depositor TM Chu
Passage History
The original tumor was passaged once in female BALB/c mice, and the resulting tumors were harvested and passaged in vitro for six months.
Year of Origin 1983
References

Capone PM, et al. Immunotherapy in a spontaneously developed murine mammary carcinoma with syngeneic monoclonal antibody. Cancer Immunol. Immunother. 25: 93-99, 1987. PubMed: 3664534

Chao TY, Chu TM. Characterization of a new spontaneously developed murine mammary adenocarcinoma in syngeneic BALB/c hosts. In Vitro Cell. Dev. Biol. 25: 621-626, 1989. PubMed: 2666384

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Capone PM, et al. Immunotherapy in a spontaneously developed murine mammary carcinoma with syngeneic monoclonal antibody. Cancer Immunol. Immunother. 25: 93-99, 1987. PubMed: 3664534

Chao TY, Chu TM. Characterization of a new spontaneously developed murine mammary adenocarcinoma in syngeneic BALB/c hosts. In Vitro Cell. Dev. Biol. 25: 621-626, 1989. PubMed: 2666384