JC (ATCC® CRL-2116)

Organism: Mus musculus, mouse  /  Cell Type: Epithelial-like  /  Tissue: mammary gland  /  Disease: adenocarcinoma

Permits and Restrictions

View Permits

Organism Mus musculus, mouse
Tissue mammary gland
Cell Type Epithelial-like
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma
Age 1.5 years
Gender Female
Strain BALB/cRos
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 40; range = 26 to 100
Derivation
JC is an epithelial-like cell line established in 1983 from a spontaneous primary mammary adenocarcinoma along the milk line.
Clinical Data
female
The original tumor was passaged once in female BALB/c mice, and the resulting tumors were harvested and passaged in vitro for six months.
Tumorigenic Yes
Effects
Yes, produces tumors with papillary adenocarcinoma morphology in BALB/c mice
Comments
Electron microscopic examination detected the presence of cellular tonofilaments and desmosomes.
No type B or C virus particles were detected, although intracisternal A particles were observed occasionally.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution and remove trypsin. Add fresh trypsin solution and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Cryopreservation
Culture medium, 95%; DMSO, 5%
Population Doubling Time 12 to 16 hrs
Name of Depositor TM Chu
Passage History
The original tumor was passaged once in female BALB/c mice, and the resulting tumors were harvested and passaged in vitro for six months.
Year of Origin 1983
References

Capone PM, et al. Immunotherapy in a spontaneously developed murine mammary carcinoma with syngeneic monoclonal antibody. Cancer Immunol. Immunother. 25: 93-99, 1987. PubMed: 3664534

Chao TY, Chu TM. Characterization of a new spontaneously developed murine mammary adenocarcinoma in syngeneic BALB/c hosts. In Vitro Cell. Dev. Biol. 25: 621-626, 1989. PubMed: 2666384

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Capone PM, et al. Immunotherapy in a spontaneously developed murine mammary carcinoma with syngeneic monoclonal antibody. Cancer Immunol. Immunother. 25: 93-99, 1987. PubMed: 3664534

Chao TY, Chu TM. Characterization of a new spontaneously developed murine mammary adenocarcinoma in syngeneic BALB/c hosts. In Vitro Cell. Dev. Biol. 25: 621-626, 1989. PubMed: 2666384