NMuMG (ATCC® CRL-1636)

Organism: Mus musculus, mouse  /  Tissue: mammary gland  / 

Organism Mus musculus, mouse
Tissue mammary gland
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Gender female
Strain NAMRU
Applications
This cell line is a suitable transfection host
Clinical Data
female
Tumorigenic Yes
Effects
Yes, benign cystadenomas are produced in isogenic mice
Comments
The cells do not express detectable retroviruses.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 10 mcg/ml insulin, 90%; fetal bovine serum, 10%
Subculturing
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Name of Depositor RB Owens
References

Owens RB. Glandular epithelial cells from mice: a method for selective cultivation. J. Natl. Cancer Inst. 52: 1375-1378, 1974. PubMed: 4363715

Owens RB, et al. Epithelial cell cultures from normal glandular tissue of mice. J. Natl. Cancer Inst. 53: 261-269, 1974. PubMed: 4366196

Yingling JM, et al. Mammalian dwarfins are phosphorylated in response to transforming growth factor beta and are implicated in control of cell growth. Proc. Natl. Acad. Sci. USA 93: 8940-8944, 1996. PubMed: 8799132

Basic Documentation
References

Owens RB. Glandular epithelial cells from mice: a method for selective cultivation. J. Natl. Cancer Inst. 52: 1375-1378, 1974. PubMed: 4363715

Owens RB, et al. Epithelial cell cultures from normal glandular tissue of mice. J. Natl. Cancer Inst. 53: 261-269, 1974. PubMed: 4366196

Yingling JM, et al. Mammalian dwarfins are phosphorylated in response to transforming growth factor beta and are implicated in control of cell growth. Proc. Natl. Acad. Sci. USA 93: 8940-8944, 1996. PubMed: 8799132