C127I (ATCC® CRL-1616)

Organism: Mus musculus, mouse  /  Tissue: mammary gland  / 

Permits and Restrictions

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Organism Mus musculus, mouse
Tissue mammary gland
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Gender female
Strain RIII
Applications

Useful for visualization of sarcoma virus-induced foci and for an in vitro quantitative assay for bovine papilloma virus.

Host for transfection with plasmids containing bovine papilloma virus DNA.

C127I cells are suitable hosts for transformation with bovine papilloma virus DNA plasmids (ATCC 37110, ATCC 37111, ATCC 37112, ATCC 39475)

Storage Conditions liquid nitrogen vapor phase
Derivation
Non-transformed clonal line derived from a mammary tumor of an RIII mouse.
Comments Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium

Dulbecco's modified Eagle's medium with 4.5 g/L glucose; 90%; fetal bovine serum, 10%

Phenol Red is a structural mimic for estrogen. Therefore the estrogenic activity of phenol red should be considered in any studies that utilize estrogen-responsive cells in culture (RefBerthois Y, et al. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc. Natl. Acad. Sci. USA 83: 2496–2500, 1986. PubMed: 3458212). The Phenol Red-free version of DMEM, is ATCC catalog No. 30-2601.

Subculturing
Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature (or 37°C) until cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:15 is recommended
Medium Renewal: 2 to 3 times per week
Name of Depositor PM Howley
References

Sarver N, et al. Bovine papilloma virus deoxyribonucleic acid: a novel eucaryotic cloning vector. Mol. Cell. Biol. 1: 486-496, 1981. PubMed: 6100967

Lowy DR, et al. Helper-independent tranformation by unintegrated Harvey sarcoma virus DNA. J. Virol. 26: 291-298, 1978. PubMed: 26810

Dvoretzky I, et al. A quantitative in Vitro focus assay for bovine papilloma virus. Virology 103: 369-375, 1980. PubMed: 6247821

Taylor GA, et al. Identification of anovel GTPase, the inducibly expresed GTPase, that accumulates in response to interferon gamma. J. Biol. Chem. 271: 20399-20405, 1996. PubMed: 8702776

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Sarver N, et al. Bovine papilloma virus deoxyribonucleic acid: a novel eucaryotic cloning vector. Mol. Cell. Biol. 1: 486-496, 1981. PubMed: 6100967

Lowy DR, et al. Helper-independent tranformation by unintegrated Harvey sarcoma virus DNA. J. Virol. 26: 291-298, 1978. PubMed: 26810

Dvoretzky I, et al. A quantitative in Vitro focus assay for bovine papilloma virus. Virology 103: 369-375, 1980. PubMed: 6247821

Taylor GA, et al. Identification of anovel GTPase, the inducibly expresed GTPase, that accumulates in response to interferon gamma. J. Biol. Chem. 271: 20399-20405, 1996. PubMed: 8702776